|Liquid nitrogen cooled cylinders are metallic dewars filled with cryogenic materials which are used to preserve fluid contained inside. Pierce Hawthorne had a seminal idea and purchased several of these items to be given as bequeathals to his study group once he passed away. For Pierce, customizing the cylinders for the group members became a labor of love. With his own hands he worked hard to put a little bit of himself into each canister. Although it was undoubtedly a pleasurable experience, the extra efforts he went to ironically and ultimately proved fatal to him. Pierce had hoped his friends would use his gifts to possibly breed an "army of geniuses". The Liquid nitrogen cooled cylinders first appearance was in the Season Five episode "Cooperative Polygraphy".|
|In the study group's fifth year at Greendale, Pierce Hawthorne passed away. As per instructions in his will, a private inquest into his death was led by a Mr. Stone after his funeral. Once the study group was finished being interrogated through polygraphy, Stone bestowed some final words and gifts Pierce had left for his friends. Along with a personal bequeathal they also each received Liquid nitrogen cooled cylinders Pierce prepared for them before his passing. Contained inside each one was what Pierce referred to as his "hyper virile" sperm. The first to recieve one of these items was Britta. She had been bequeathed a iPod nano along with some advice about not hating herself so much.
|Shirley was then given a canister of her own along with a timeshare Pierce owned in Florida. Annie received one next and after being told by Stone that she was always Pierce's favorite got a tiara he tried to give her years ago. Stone turned to Jeff and questioned his sexuality before giving him a bottle of 40 year old scotch and his obligatory cylinder. Abed was the only one in the group not to receive something else besides Pierce's sperm as Stone told him Pierce always thought he was crazy and never understood anything he said. Troy was the last to receive Pierce's "donation" and expressed his disappointment at his bequeathal.
|Stone told Troy that Pierce always felt he had the heart of a hero. He then revealed that in addition Pierce left Troy his Hawthorne Wipes shares valued at 14.3 million dollars. The only stipulation was that he complete a task Pierce was given by his father when he was Troy's age: to sail around the world. Jeff told Troy that he could easily contest the provision but Troy readily accepted the terms of the will. Afterwards, Mr. Stone went out drinking with the study group and was asked by Britta how Pierce died. Stone explains that the cause was actually dehydration due to Pierce having filled up all the liquid nitrogen cooled cylinders ("Cooperative Polygraphy").